Anti-mitotic activity towards sea urchin eggs of dichloromethane fraction obtained from Allamanda schottii Pohl (Apocynaceae)

Revista Brasileira de Farmacognosia

Oliveira, Márcia R.¹  Schmidt, Dionezine F. Navarro¹  Universidade Estadual de Ponta Grossa, Ponta Grossa, Brasil¹  Universidade Federal da Paraíba, João Pessoa, Brasil¹  Sousa, Louisa M. A.¹  Universidade Federal da Paraíba, Pessoa, Brasil¹  Monte Neto, Rubens L.¹ 

关键词: Allamanda schottii;    sea urchin;    anti-mitotic activity;    anti-tumor.;   

DOI  :  10.1590/S0102-695X2009000300002

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Allamanda (Apocynaceae) is a genus of climbing shrubs known for producing compounds with a range of biological activities. Previous works have shown the anti-proliferative effect of the ethanolic extract of Allamanda schottii on leukemic cells. The present work was conducted to evaluate the effects of dichloromethane fraction, obtained from Allamanda schottii, on sea urchin Echinometra lucunter eggs, as a multicellular model for evaluating anti-tumor activity. Our results show an inhibition of sea urchin development in a dose-dependent manner in the presence of dichloromethane fraction. The IC50 values for first and third cleavage and blastulae stage were 103.7 µg/mL, 33.1 µg/mL and 10.2 µg/mL, respectively. These results also demonstrate the cumulative effect of this fraction on sea urchin embryos. In the present work, the expressive anti-mitotic activity of dichloromethane fraction towards sea urchin eggs, a multicellular model, reinforces the anti-tumor potential of the Allamanda schotti.

【 授权许可】

Unknown