Anti-mitotic activity towards sea urchin eggs of dichloromethane fraction obtained from Allamanda schottii Pohl (Apocynaceae)

Revista Brasileira de Farmacognosia

Louisa M. A. Sousa²  Rubens L. Monte Neto¹  Dionezine F. Navarro Schmidt¹  Márcia R. Oliveira² 

[1] ,Universidade Federal da Paraíba Departamento de Biologia Molecular João Pessoa PB ,Brasil

关键词: Allamanda schottii;    sea urchin;    anti-mitotic activity;    anti-tumor;    Allamanda schottii;    ouriço do mar;    atividade anti-mitótica;    anti-tumoral;   

DOI  :  10.1590/S0102-695X2009000300002

来源: SciELO

【 摘 要 】

Allamanda (Apocynaceae) is a genus of climbing shrubs known for producing compounds with a range of biological activities. Previous works have shown the anti-proliferative effect of the ethanolic extract of Allamanda schottii on leukemic cells. The present work was conducted to evaluate the effects of dichloromethane fraction, obtained from Allamanda schottii, on sea urchin Echinometra lucunter eggs, as a multicellular model for evaluating anti-tumor activity. Our results show an inhibition of sea urchin development in a dose-dependent manner in the presence of dichloromethane fraction. The IC50 values for first and third cleavage and blastulae stage were 103.7 µg/mL, 33.1 µg/mL and 10.2 µg/mL, respectively. These results also demonstrate the cumulative effect of this fraction on sea urchin embryos. In the present work, the expressive anti-mitotic activity of dichloromethane fraction towards sea urchin eggs, a multicellular model, reinforces the anti-tumor potential of the Allamanda schotti.

【 授权许可】

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