Antioxidant activity of Nelumbo nucifera (Gaertn) flowers in isolated perfused rat kidney

Revista Brasileira de Farmacognosia

Krishnamoorthy, Gayathri¹  Rajamanickam, Victor G.¹  Ravindhran, Dhevi¹  Shabi, Mohamed M.¹  Chellappan, David Raj¹  Joseph, Jipnomon¹  Uthrapathy, Subashini¹  Dubey, Govinda P.¹  Sastra University, Tamilnadu, India¹ 

关键词: Nelumbo nucifera;    Nymphaceae;    Fenton mixture;    malondialdehyde;    GOT;    isolated perfused kidney.;   

DOI  :  10.1590/S0102-695X2009000200008

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Standardization of induction of oxidative stress with Fenton mixture in isolated perfused rat kidney and evaluation of antioxidant effect of Nelumbo nucifera flowers in isolated oxidatively stressed rat kidney are carried out in present study. Six groups of isolated perfused rat kidney were used for the present study. Group I kidneys were received vehicle. Group II kidneys were oxidatively stressed with Fenton mixture. Group III and IV animals were treated with two graded doses of extract after the administration of Fenton mixture. Group V and VI kidneys were perfused with two graded doses of extract without Fenton mixture. Fenton mixture was found to cause oxidative stress with significant difference of malondialdehyde (TBARS), Reduced glutathione (GSH), Glutathione peroxidase (GPx), catalase, Glutamate Oxaloacetate Transaminase (GOT) and Glutamate Pyruvate Transaminase (GPT) (p<0.05) in diseased rats. On treating animals with extract, the oxidative stress was decreased with increase in antioxidants and the marker enzymes were found to maintain the normal level. This effect of extract was found to be dose dependent. In conclusion, Fenton mixture developed the oxidative stress in isolated perfused rat kidney and Nelumbo nucifera flowers extract exhibits antioxidant activity in that oxidatively stressed isolated perfused kidney.

【 授权许可】

Unknown