Ultraviolet spectroscopy method for coumarin quantification in Mikania glomerata Sprengel syrup

Revista Brasileira de Farmacognosia

Universidade do Sul de Santa Catarina, Tubarão, Brasil¹  Kanis, Luiz A.¹  Gomes, Víctor S.¹  Kulkamp, Irene C.¹  Silva, Cleinils R. da¹ 

关键词: Mikania glomerata;    Asteraceae;    Coumarin;    Spectrophotometer;    Methylparaben;    Syrup.;   

DOI  :  10.1590/S0102-695X2008000400017

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

A spectrophotometric procedure for coumarin determination in Mikania glomerata Sprengel (guaco) syrup is described in this work. Due to the high number of constituents in guaco syrups, the coumarin was extracted with apolar extractors (chloroform and hexane), in which chloroform was selected, because of its higher capacity of extraction. After the solvent choice, the wavelength at 320 nm, region where there is the lower interference of syrup constituents, was selected. The calibration curve showed linearity, R2 of 0.99978. The spectrophotometric assay of coumarin in three samples of Mikania glomerata Sprengel syrup showed accuracy compared with the HPLC method. The results presented suggest that the spectrophotometric method may be useful for the quantitative analysis of coumarin in Mikania glomerata Sprengel syrup.

【 授权许可】

Unknown