Evaluation of antiviral activity and determination of the chromatographic profile of Hippeastrum glaucescens (Martius) Herbert (Amaryllidaceae)

Revista Brasileira de Farmacognosia

Universidade Regional Integrada, Erechim¹  Dutilh, J.¹  Henriques, A. T.¹  Zuanazzi, J. A. S.¹  Universidade Federal do Rio Grande do Sul, Porto Alegre¹  Montanha, J.A.¹  Seben, C.¹  Sobral, M.¹  Hofmann JR, A. E.¹  Universidade Estadual de Campinas, Campinas¹ 

关键词: Hippeastrum glaucescens;    Amaryllidaceae;    isoquinoline alkaloids;    chromatographic profile;    antiviral activity.;   

DOI  :  10.1590/S0102-695X2004000100002

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Plants of Amaryllidaceae are characterized by isoquinoline alkaloids. Since the first study with Amaryllidaceae alkaloids in 1877, a large number of these plants have been chemically investigated. These compounds have shown a wide range of biological activities such as: antiviral, cytotoxic, antitumoral and analgesic. In this work, the dichloromethane (CH2Cl2) extracts obtained from different parts of the Hippeastrum glaucescens (Martius) Herbert (bulbs, roots, leaves and flowers) and the isolated alkaloids lycorine, tazettine and pretazettine were analyzed by a chromatographic method (TLC) and tested for antiviral activity. The extraction of alkaloids from bulbs, roots, leaves and flowers of H. glaucescens was performed by classic methods and yields 0.53%, 0.81%, 0.29% and 0.12%, respectively. Through TLC, bulbs and roots revealed similar chromatographic profiles and lycorine, tazettine and pretazettine were found in all the parts analyzed. The CH2Cl2-A and CH2Cl2-B extracts from each part of the plant and the isolated alkaloids (lycorine, tazettine and pretazettine) did not inhibit the HSV-1 strain KOS replication, when evaluated through the inhibition of cytophatic viral effect.

【 授权许可】

Unknown