Myonecrosis induced in rat by a myotoxin isolated from the venom of Bothrops neuwiedifrom Alfenas, MG

Revista Brasileira de Farmacognosia

Leite, V.L.¹  Gouvêa, C.M. C. P.¹  Lúcia, M.¹  Andrade, M.F.¹  Universidade de Alfenas, Alfenas¹ 

关键词: Bothrops neuwiedi;    HPLC;    myonecrosis;    myotoxin;   

DOI  :  10.1590/S0102-695X2001000100003

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Bothrops venoms are composed by several protein fractions. Among these fractions there are myotoxins which induce an important muscle lesion. The purification of this component involves some steps, although providing a pure material, is time consuming. In the present study, we describe a quick method for myotoxin fraction isolation from the venom of Bolhrops nsuwiedi using one-step high performance liquid chromatography (HPLC). The complete procedure took 30 min. The pur6+y of the two myotoxic fractions isolated was assessed by SDS-PAGE. Upon i.m. injection into rat tibialis anterior, both toxins induced early morphological changes, indicating that the plasma membrane was the first cellular structure affected. Afterwards, the lesion was typically myonecrotic and inflammatory infiltrate was present.

【 授权许可】

Unknown