ELISA Detection of Francisella tularensis using Polyclonaland Monoclonal Antibodies

Defence Science Journal

Miroslav Pohanka¹  Oto Pavlis²  Michal Kroca² 

[1] University of Defence, Hradec Kralove;Central Military Institute of Health, Techonin

关键词: Tularemia;    Francisella tularensis;    ELISA;    biowarfare agent;    immunodetection;    enzyme-linkedimmunosorbent assay;    antibodies;    pathogen detection;   

DOI  :  

学科分类：社会科学、人文和艺术（综合）

来源: Defence Scientific Information & Documentation Centre

【 摘 要 】

The mouse monoclonal and polyclonal antibodies were produced for the detection of intracellular pathogenand potential warfare agent Francisella tularensis. Antibody titers obtained were 1:640 for polyclonal antibodiesand 1:320 for monoclonal antibodies. Both antibodies were used in the indirect enzyme-linked immunosorbentassay (ELISA) found to detect F. tularensis whole cells. The limit of detection was 5.4×106 CFU/ml for polyclonalantibodies and 6.9×106 CFU/ml for monoclonal antibodies. The value sample could  be distinguished from anyconcentration of another gram-negative bacterium: Escherichia coli. Defence Science Journal, 2008, 58(5), pp.698-702 , DOI:http://dx.doi.org/10.14429/dsj.58.1693

【 授权许可】

Unknown