Candida phyllophila sp. nov. and Candida vitiphila sp. nov., two novel yeast species from grape phylloplane in Thailand

The Journal of General and Applied Microbiology

Rungluk Kaewwichian¹  Savitree Limtong¹ 

[1] Department of Microbiology, Faculty of Science, Kasetsart University

关键词: Candida phyllophila sp. nov.;    Candida vitiphila sp. nov.;    phylloplane;    Thailand;   

DOI  :  10.2323/jgam.59.191

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Three strains (K59T, K60 and K70 T) representing two novel yeast species were isolated from the external surface of leaves of different wine grape (Vitis vinifera) plants, which were collected from the Kanchanaburi Research Station (N14°07′15.1″ E099°19′05.6″), Wang Dong Sub-district, Mueang District, Kanchanaburi Province, Thailand, by an enrichment technique. The sequences of the D1/D2 domain of the large subunit (LSU) rRNA gene of two strains (K59T and K60) were identical and differed from that of strain K70T. In terms of pairwise sequence similarity of the D1/D2 domain, the closest species to the three strains was Candida asparagi but with 2.3% nucleotide substitutions for strains K59T and K60, and 2.1% nucleotide substitutions for strain K70T. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and the sequence analysis of the D1/D2 domain of the large subunit (LSU) rRNA gene, the three strains were assigned to be two novel Candida species. Two strains (K59T and K60) were assigned as Candida phyllophila sp. nov. (type strain K59T=BCC 42662T=NBRC 107776T=CBS 12671T). Candida vitiphila sp. nov. is proposed for strain K70T (=BCC 42663T=NBRC 107777T=CBS 12672T).

【 授权许可】

Unknown