Practical use of CMC-amended rhizobial inoculant for Mucuna pruriens cultivation to enhance the growth and protection against Macrophomina phaseolina

The Journal of General and Applied Microbiology

Abhinav Aeron²  Naveen Kumar Arora³  Dinesh Kumar Maheshwari²  Ekta Khare¹ 

[1] Department of Microbiology, Institute of Biosciences and Biotechnology, CSJM University;Department of Botany and Microbiology, Gurukul Kangri University;Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University

关键词: Bradyrhizobium;    carboxymethyl cellulose;    Ensifer meliloti;    Macrophomina phaseolina;    Mucuna pruriens;    rhizobia;    sawdust;   

DOI  :  10.2323/jgam.58.121

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

In many parts of the world Mucuna pruriens is used as an important medicinal, forage and green manure crop. In the present investigation the effect of the addition of CMC in carrier during development of bioformulation on shelflife, plant growth promotive and biocontrol activity against Macrophomina phaseolina was screened taking M. pruriens as a test crop. Ensifer meliloti RMP6Ery+Kan+ and Bradyrhizobium sp. BMP7Tet+Kan+ (kanamycin resistance engineered by Tn5 transposon mutagenesis) used in the study showed production of siderophore, IAA, solubilizing phosphate and biocontrol of M. phaseolina. RMP6Ery+Kan+ also showed ACC deaminase activity. The survival of both the strains in sawdust-based bioformulation was enhanced with an increase in the concentration of CMC from 0 to 1%. At 0% CMC Bradyrhizobium sp. BMP7Tet+Kan+ showed more increase in nodule number/plant (500.00%) than E. meliloti RMP6Ery+Kan+ (52.38%), over the control in M. phaseolina-infested soil. There was 185.94% and 59.52% enhancement in nodule number/plant by RMP6Ery+Kan+ and BMP7Tet+Kan+ with an increase in the concentration of CMC from 0% to 1% in the bioformulations. However further increase in concentration of CMC did not result in enhancement in survival of either the strains or nodule number/plant.

【 授权许可】

Unknown