A simple method for culture and stable maintenance of giant spheroplasts from the yeast Saccharomyces cerevisiae

The Journal of General and Applied Microbiology

Isamu Miyakawa²  Tomohiro Kotani¹  Taihei Fukudomi² 

[1] Department of Biology, Faculty of Science, Yamaguchi University;Department of Environmental Science and Engineering, Graduate School of Science and Engineering,Yamaguchi University

关键词: DAPI;    2-deoxyglucose;    giant spheroplast;    mitochondrial nucleoids;    Saccharomyces cerevisiae;    yeast;   

DOI  :  10.2323/jgam.57.177

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

When spheroplasts of the yeast Saccharomyces cerevisiae are cultured in liquid medium containing osmotic stabilizer, they undergo nuclear division and growth without cell division, resulting in the formation of giant spheroplasts with multinuclei. In this study, we report a simple method for the culture and stable maintenance of giant spheroplasts. The selection of culture media and cell concentration was found to be important for the growth and maintenance of giant spheroplasts. Among the conditions that we tested, static culture in a synthetic Burkholder’s medium in 96-well U-bottomed culture plates was most effective. Under appropriate conditions, we could maintain giant spheroplasts for more than 6 days without proliferation of whole cells or marked lysis. The average diameter of spheroplasts can vary from 16 to 53μm, depending on their initial concentration.

【 授权许可】

Unknown