Simple detection of a yeast mitochondrial DNA-binding protein, Abf2p, on SDS-DNA gels

The Journal of General and Applied Microbiology

Isamu Miyakawa¹  Takahiro Jyozaki¹  Hiroshi Sato¹  Takao Umezaki¹  Yoko Kitamura¹ 

[1] Department of Biological Science, Faculty of Science, Yamaguchi University

关键词: Abf2p;    DNA-binding protein;    mitochondria;    mitochondrial nucleoids;    Saccharomyces cerevisiae;    yeast;   

DOI  :  10.2323/jgam.46.311

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Abf2p, a mitochondrial DNA-binding protein of yeast Saccharomyces cerevisiae, was selectively detected among mitochondrial nucleoid proteins by SDS-DNA polyacrylamide gel electrophoresis (SDS-DNA PAGE) followed by ethidium bromide staining. This method is simple and specific for the detection of Abf2p, and it may be used to identify an Abf2p-like protein that is present in mitochondrial nucleoids from other yeasts.

【 授权许可】

Unknown