期刊论文详细信息
The Journal of General and Applied Microbiology | |
Simple detection of a yeast mitochondrial DNA-binding protein, Abf2p, on SDS-DNA gels | |
Isamu Miyakawa1  Takahiro Jyozaki1  Hiroshi Sato1  Takao Umezaki1  Yoko Kitamura1  | |
[1] Department of Biological Science, Faculty of Science, Yamaguchi University | |
关键词: Abf2p; DNA-binding protein; mitochondria; mitochondrial nucleoids; Saccharomyces cerevisiae; yeast; | |
DOI : 10.2323/jgam.46.311 | |
学科分类:微生物学和免疫学 | |
来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation | |
【 摘 要 】
Abf2p, a mitochondrial DNA-binding protein of yeast Saccharomyces cerevisiae, was selectively detected among mitochondrial nucleoid proteins by SDS-DNA polyacrylamide gel electrophoresis (SDS-DNA PAGE) followed by ethidium bromide staining. This method is simple and specific for the detection of Abf2p, and it may be used to identify an Abf2p-like protein that is present in mitochondrial nucleoids from other yeasts.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912010138683ZK.pdf | 216KB | download |