A new, simple, rapid test for detection of DNase activity of microorganisms: DNase Tube test

The Journal of General and Applied Microbiology

Djursun Karasartova¹  Saeid Barkar¹  M. Kerem Calgin¹  Emel Elyürek¹  T. Murat Özsan¹  Fikret Sahin¹  Devran Gerceker¹  Mehmet KIyan¹ 

[1] Microbiology Department, School of Medicine, Ankara University

关键词: DNase test;    DNase activity;    Staphylococcus;   

DOI  :  10.2323/jgam.55.291

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The DNase test is a simple, economical method that has traditionally been used as a supplemental test to identify pathogenic Staphylococcus. This test also aids in the differentiation of closely-related genera within the Klebsiella-Enterobacter-Serratia division of Enterobacteriaceae and several other pathogens, including screening of C. diphtheriae. Currently DNase activity of microorganisms was tested using DNase agar plate methods. These tests have some drawbacks including the necessity of the extensive time to see the results of DNase activity of bacteria. In here, we developed a new method which is simple, rapid, inexpensive and applicable to examine DNase activity of any bacteria. In this method, simply, bacteria is added to the broth medium containing DNA and followed for the DNA degradation caused by the DNase of bacteria by running in agarose gel. This method we called DNase Tube test showed DNA degradation as fast as in half an hour depending on the DNase activity of the bacteria.

【 授权许可】

Unknown