Characterization of DNase activity and gene in Streptococcus suis and evidence for a role as virulence factor

BMC Research Notes

Daniel Grenier²  Marcelo Gottschalk¹  Steve J Charette²  Katy Vaillancourt³  Laetitia Bonifait³  Bruno Haas³ 

[1] Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada;Centre de Recherche en Infectiologie Porcine et Avicole (CRIPA), Fonds de Recherche du Québec - Nature et Technologies (FRQNT), Saint-Hyacinthe, Quebec, Canada;Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire, Université Laval, 2420 Rue de la Terrasse, Quebec City, Quebec G1V 0A6, Canada

关键词: SsnA;    Virulence factor;    DNase activity;    nuclease;    Streptococcus suis;   

Others  :  826310
DOI  :  10.1186/1756-0500-7-424

 received in 2014-04-07, accepted in 2014-06-27,  发布年份 2014

【 摘 要 】

Background

The Gram-positive bacterium Streptococcus suis serotype 2 is an important swine pathogen and emerging zoonotic agent. Multilocus sequence typing allowed dividing S. suis serotype 2 into sequence types (STs). The three major STs of S. suis serotype 2 from North America are 1 (most virulent), 25 (intermediate virulence) and 28 (less virulent). Although the presence of DNase activity in S. suis has been previously reported, little data is available. The aim of this study was to investigate DNase activity in S. suis according to STs, to characterize the activity and gene, and to provide evidence for a potential role in virulence.

Results

We showed that ST1 and ST28 strains exhibited DNase activity that was absent in ST25 strains. The lack of activity in ST25 isolates was associated with a 14-bp deletion resulting in a shifted reading frame and a premature stop codon. The DNase of S. suis P1/7 (ST1) was cell-associated and active on linear DNA. A DNase-deficient mutant of S. suis P1/7 was found to be less virulent in an amoeba model. Stimulation of macrophages with the DNase mutant showed a decreased secretion of pro-inflammatory cytokines and matrix metalloproteinase-9 compared to the parental strain.

Conclusions

This study further expands our knowledge of S. suis DNase and its potential role in virulence.

【 授权许可】

   
2014 Haas et al.; licensee BioMed Central Ltd.

【 图 表 】

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