Medium optimization by orthogonal array and response surface methodology for cholesterol oxidase production by Streptomyces lavendulae NCIM 2499

The Journal of General and Applied Microbiology

Jyoti Kishenkumar¹  Uday S. Annapure¹  Awadesh K. Chauhan¹  Shrikan A. Survase¹ 

[1] Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai

关键词: cholesterol oxidase;    orthogonal array;    response surface methodology;    Streptomyces lavendulae NCIM 2499;   

DOI  :  10.2323/jgam.55.171

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

This paper deals with the optimization of culture conditions for the production of cholesterol oxidase (COD) by Streptomyces lavendulae NCIM 2499 using the one-factor-at-a-time method, orthogonal array method and response surface methodology (RSM) approaches. The one-factor-at-a-time method was adopted to investigate the effects of medium components (i.e. carbon and nitrogen) and environmental factors (i.e. initial pH) on biomass growth and COD production. Subsequently, an L12 orthogonal matrix was used to evaluate the significance of glycerol, soyabean meal, malt extract, K2HPO4, MgSO4 and NaCl. The effects of media components were ranked according to their effects on the production of COD as malt extract > soyabean meal > K2HPO4 > NaCl > MgSO4 > glycerol. The subsequent optimization of the four most significant factors viz. malt extract, soyabean meal, K2HPO4 and NaCl, was carried out by employing a central composite rotatable design (CCRD) of RSM. There was a 2.48-fold increase in productivity of COD as compared to the unoptimized media by using these statistical approaches.

【 授权许可】

Unknown