Phylogeny of γ-polyglutamic acid-producing Bacillus strains isolated from a fermented locust bean product manufactured in West Africa

The Journal of General and Applied Microbiology

Jomkhwan Meerak¹  Mika Miyashita²  Yasuyoshi Nakagawa²  Hajime Sato²  Yasutaka Tahara¹  Pattaraporn Yukphan¹ 

[1] Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University;Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation

关键词: Bacillus;    dawadawa;    fermented locust beans;    phylogeny;    γ-polyglutamic acid;    rpoB and fus sequences;    16S rDNA sequences;   

DOI  :  10.2323/jgam.54.159

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Twenty-five Bacillus strains capable of producing γ-polyglutamic acid (PGA) were isolated from fermented locust bean products manufactured in the savanna area of Ghana. To clarify the phylogeny of these PGA-producing strains, phylogenetic analyses based on sequences of 16S rDNA, rpoB (RNA polymerase β-subunit) and fus (elongation factor G) genes were performed. A phylogenetic tree based on 16S rDNA indicated that ten isolates were clustered in the same group of Bacillus subtilis. Another ten isolates were located in the cluster of B. amyloliquefaciens, and the remaining isolates were identified as B. pumilus (three isolates) and B. licheniformis (two isolates), respectively. Phylogenetic trees based on the partial sequences of rpoB and fus genes were similar to the phylogeny based on 16S rDNA sequences. Thirty-four strains in 27 species belonging to the genus Bacillus and its neighbors were also investigated for PGA production. It was found that PGA was produced by B. amyloliquefaciens NBRC 14141 and NBRC 15535T, B. atrophaeus NBRC 15539T, B. licheniformis NBRC 12107, B. mojavensis NBRC 15718T, B. pumilus NBRC 12094, B. subtilis NBRC 16449, and Lysinibacillus sphaericus NBRC 3525. Except for L. sphaericus, the above Bacillus species are very closely related in phylogeny, indicating that PGA-producing Bacillus strains constitute a cluster.

【 授权许可】

Unknown