Microbial analysis of a composted product of marine animal resources and isolation of bacteria antagonistic to a plant pathogen from the compost

The Journal of General and Applied Microbiology

Hisashi Miyamoto³  Hirokuni Miyamoto¹  Shin-ichiro Oka²  Mitsuyo Hirai⁷  Jirou Matsumoto⁶  Chie Niisawa²  Kenichi Mori¹  Yoshifumi Kumagai⁴  Hiroaki Kodama⁵ 

[1] Japan Eco-science Co., Ltd.;Graduate School of Science and Technology, Chiba University;Miroku Co., Ltd.;Keiyo Plant Engineering, Co., Ltd.;Graduate School of Horticulture, Chiba University;Keiyo Gas Co., Ltd.;Center for Advanced Materials Analysis (Suzukakedai), Technical Department, Tokyo Institute of Technology

关键词: DGGE;    iturin;    plant growth-promoting rhizobacteria;    16S rRNA gene;    soil fertilizer;    thermophilic bacteria;   

DOI  :  10.2323/jgam.54.149

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

A composting product of marine animal resources has been used as a fertilizer and a soil conditioner in Japan. This compost was produced by a repeated fed-batch fermentation system with three successive aerobic bioreactors. Composting temperature reached about 75°C without heating. The bacterial diversity in this compost was investigated by denaturing gradient gel electrophoresis (DGGE) and sequence determination of the V3 region in the 16S rRNA genes. The sequence analysis showed that a majority of retrieved sequences corresponded to those of Bacillaceae, and we frequently found sequences similar to the 16S rDNA sequences of Bacillus thermocloacae and Bacillus thermoamylovorans. In addition, a bacterium antagonistic to a Fusarium strain was isolated from the compost. The isolate (Bacillus sp. NP-1) produced an antifungal compound, iturin A. These results suggest that this compost serves as a valuable source of plant growth-promoting rhizobacteria including the antifungal bacteria.

【 授权许可】

Unknown