Degradation of phenanthrene on plant leaves by phyllosphere bacteria

The Journal of General and Applied Microbiology

Karen Waight¹  Ekawan Luepromchai²  Onruthai Pinyakong² 

[1] International Postgraduate Programs in Environmental Management, Graduate School, Chulalongkorn University;Department of Microbiology, Faculty of Science, Chulalongkorn University

关键词: air pollutants;    biodegradation;    phenanthrene;    phyllosphere bacteria;   

DOI  :  10.2323/jgam.53.265

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The activity of phyllosphere bacteria in the degradation of phenanthrene was investigated as a mechanism for the removal of atmospheric phenanthrene after its deposition on plant leaves. Initially, leaf samples of six plant species were collected from two roadsides in Bangkok to determine the presence of phenanthrene-degrading bacteria. The numbers of phenanthrene-degrading phyllosphere bacteria were varied and ranged from 3.5×104 to 1.95×107 CFU/g, in which the highest number was found from Ixora sp. Further studies were carried out in the laboratory by spraying phenanthrene on Ixora sp. leaves and then monitoring the amount of deposited phenanthrene and number of phenanthrene-degrading bacteria after incubation. The results showed that the amount of phenanthrene was significantly reduced on leaves containing phenanthrene-degrading bacteria. These were detected along with a rapid increase in the number of bacteria on leaves. The results indicated that many phyllosphere bacteria could utilize phenanthrene to support their growth and thereby reduce the amount of deposited phenanthrene on leaf surfaces. Several phenanthrene-degrading bacteria were later isolated from the leaves and identified with a high 16S rDNA sequence similarity to the genera Pseudomonas, Microbacterium, Rhizobium, and Deinococcus.

【 授权许可】

Unknown