Isolation and characterization of mesophilic cellulose-degrading bacteria from flower stalks-vegetable waste co-composting system

The Journal of General and Applied Microbiology

Hong-Tao Wang¹  Shi-Jian Yang¹  Wen-Jing Lu¹  Yong-Feng Nie¹  Zhi-Chao Wang¹ 

[1] Department of Environmental Science and Engineering, Tsinghua University

关键词: cellulose-degrading bacteria;    characterization;    composting;    identification;   

DOI  :  10.2323/jgam.51.353

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Fifteen mesophilic bacteria with high Cx cellulase activities were isolated and purified from a mixed-culture enriched from a flower stalks-vegetable waste co-composting system. A CMCase test showed that the enzyme activity of these isolates ranged from 7.9 to 28.0 U ml−1. Although filter paper degrading capability was low in single culture, significant synergetic cellulose degradation were detected in four groups of mixed cultures, their degradation rates were 23.5%, 26.3%, 19.4% and 24.5%, respectively. Study of morphological and physiological characters of five predominant isolates which possess high CMCase and had positive effect on synergetic cellulose degradation in mixed culture system showed that two of them were closely related to Bacillus pasteurii and Bacillus cereus, whereas the rest belong to the genus Halobacillus, Aeromicrobium and Brevibacterium, respectively.

【 授权许可】

Unknown