Genetic variations in the pgm locus among natural isolates of Yersinia pestis

The Journal of General and Applied Microbiology

Ling Zhang¹  Decui Pei¹  Zhaobiao Guo¹  Zongzhong Tong³  Baizhong Cui²  Lixia Jin²  Zhizhen Qi²  Xiaoyi Wang¹  Huanming Yang³  Peitang Huang¹  Yajun Song¹  Jin Wang¹  Yanping Han¹  Min Li²  Zongmin Du¹  Jian Wang³  Ruifu Yang¹  Junhui Zhai¹  Dongsheng Zhou¹  Xin Pang¹ 

[1] Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences;Qinghai Institute for Endemic Diseases Prevention and Control;Beijing Genomics Institute, Chinese Academy of Sciences

关键词: genetic variation;    pgm locus;    plague;    Yersinia pestis;   

DOI  :  10.2323/jgam.51.11

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

A PCR-based screening method was used to study the genetic variations of the pgm locus among natural isolates of Yersinia pestis from China. Our results indicate that genetic variations in the pgm locus are well correlated with biovars of Y. pestis and plague foci, suggesting that the pgm locus plays a role in Y. pestis adaptation to its environment. The gene encoding two-component regulatory system sensor kinase became a pseudogene in all strains of biovar Orientalis due to a thymidine deletion, while it is intact in all the strains of the other biovars. Only strains from Foci H and L are the same as Yersinia pseudotuberculosis in that they have an intact transmembrane helix in the sensor kinase protein, which is lost in all the other strains because of the 18 bp in-frame deletion. The IS100 element that flanks the 3′ terminus of the pgm locus was inserted into the chromosome during the within-species microevolution of Y. pestis, which is absent in strains from Foci G, H and L and also in Y. pseudotuberculosis. This fact indicates that the strains from these three foci are of an older lineage of Chinese Y. pestis. It is this IS100 element's absence that maintained high stability of the pgm locus in the Y. pestis strains from these three foci. The IS285 element insertion in the pigmentation segment and the IS100 element insertion in the downstream flanking region of the pgm locus are only present in strains from Foci H and L. The flanking region outside the 5′ terminus of the upstream IS100 element is identical in the strains from these two foci, which is different in the other strains. All of these unique characteristics suggest that they are of a special lineage of Chinese Y. pestis.

【 授权许可】

Unknown