Ethylene glycol bis(beta-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) inhibits the growth of Escherichia coli at alkaline pH

The Journal of General and Applied Microbiology

Tomohito Kakegawa¹  Yuuko Arakawa¹  Hiroshi Kobayashi¹  Hiromi Saito¹  Tatsuya Saito¹ 

[1] Faculty of Pharmaceutical Sciences, Chiba University

关键词: alkaline pH;    calcium ions;    EGTA;    Escherichia coli;    growth;   

DOI  :  10.2323/jgam.46.127

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The growth of Escherichia coli was inhibited by ethylene glycol bis(beta-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA) in a medium of initial pH 8.8. The growth inhibition was reversed by the addition of CaCl2. E. coli could grow in the presence of EGTA at pH values below 8. The concentration of free calcium ions increases with a decrease in medium pH because of a decrease in the calcium binding capacity of EGTA. So, although the results suggest that calcium ions are essential for the growth of E. coli, the minimum concentration required is very low.

【 授权许可】

Unknown