Salt stress sensitivity of nitrogen fixation in Enterobacter agglomerans strains

The Journal of General and Applied Microbiology

Gabriele Rieder¹  Raman Rai¹ 

[1] Department of Genetics, University of Bayreuth

关键词: Enterobacter agglomerans;    nitrogenase activity;    nitrogenase synthesis;    sodium chloride;   

DOI  :  10.2323/jgam.44.365

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Two strains 333 to 339 of Enterobacter agglomerans were selected in the present study to evaluate the response of increasing concentrations of NaCl on growth, N2-fixation, and nitrogenase activity/synthesis. E. agglomerans strains 333 and 339 showed optimum growth and acetylene-reducing activity with 0.5 to 1.0% NaCl in a nitrogen-free minimal medium (NFDM) with glucose, respectively, in 28 h incubation, although both strains displayed better growth and acetylene-reducing activity with 3.0% and 2.0% NaCl after 52 h and 100 h incubation periods than the 28 h culture did. Our experiments with shiftings of salt concentrations in NFDM medium indicated that a synthesis of nitrogenase enzyme was generally more sensitive to higher concentrations of NaCl than nitrogenase activity was.

【 授权许可】

Unknown