A Class II fructose-1,6-bisphosphate aldolase from a halophilic archaebacterium Haloferax mediterranei

The Journal of General and Applied Microbiology

Wijaya Altekar¹  Sandra E. D'Souza¹ 

[1] Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre

关键词: Class II aldolase;    divalent metal;    EDTA;    enzyme purification;    fructose-1;    6-bisphosphate;    Haloferax mediterranei;    halophilic;    metal chelator;   

DOI  :  10.2323/jgam.44.235

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Fructose-1,6-bisphosphate (FBP) aldolase (EC 4.1.2.13) was purified 97-fold from a halophilic archaebacterium Haloferax mediterranei, with a specific activity of 2.8. The enzyme was characterized as a Class II aldolase on the basis of its inhibition by EDTA and other metal chelators. The enzyme had a specific requirement for divalent metal Fe2+ for activity. Sulfhydryl compounds enhanced aldolase activity.

【 授权许可】

Unknown