Experimental determination of stable carbon and nitrogen isotope fractionation between mangrove leaves and crabs

Marine Ecology Progress Series

Carolin M. Herbon¹  Inga Nordhaus¹ 

关键词: Fractionation;    Discrimination factor;    Stable isotopes;    Nitrogen;    Carbon;    Consumption rate;    Food preference;    Mangrove;   

DOI  :  10.3354/meps10421

学科分类：海洋学与技术

来源: Inter-Research

【 摘 要 】

ABSTRACT: The analysis of benthic food webs in mangrove forests is complicated by the lack of experimental investigations of consumer-diet discrimination factors (Δ¹⁵N and Δ¹³C) for most mangrove invertebrates. This study aimed to determine the fractionation of δ¹³C and δ¹⁵N between mangrove leaves and Episesarma singaporense and E. versicolor (Sesarmidae) for 90 d in Java, Indonesia. The consumption rates of both species and stable isotope fractionation of leaves from 5 plant species (Acanthus ilicifolius, Aegiceras corniculatum, Avicennia alba, Derris trifoliata, Rhizophora apiculata) were compared. Δ¹⁵N between R. apiculata leaves and muscle tissue was 5.0‰ for E. singaporense and 5.4‰ for E. versicolor after 50 d. δ¹⁵N of muscle tissue increased significantly after 50 d, most likely due to the internal recycling of nitrogen. δ¹³C did not change during the experiment, and Δ¹³C was 5.1‰ for E. singaporense and 4.1‰ for E. versicolor after 90 d. Leaves of D. trifoliata and R. apiculata were preferentially consumed, indicating their higher nutritive value. We concluded that (1) the discrimination values for mangrove crabs feeding on a leaf diet are much higher than previously assumed, which can most likely be explained by the selective assimilation of isotopic heavy carbon compounds and by metabolic cycling of non-essential amino acids; (2) the frequently used average discrimination factors are inappropriate for the study of benthic food webs in mangrove forests with a high biomass of leaf-eating crabs; and (3) E. singaporense and E. versicolor can meet their nitrogen demand by assimilating nitrogen from R. apiculata leaves and by using internal reserves for at least 50 d.

【 授权许可】

Unknown