エステラーゼアイソザイムを利用したオオムギ縞萎縮病抵抗性遺伝子の集積法

日本作物学会紀事

吉田 智彦¹  河田 尚之³  五月女 敏範² 

[1] 宇都宮大学農学部;栃木県農業試験場栃木分場;九州沖縄農業研究センター

关键词: Esterase isozyme;    エステラーゼアイソザイム;    Barley;    オオムギ;    BaYMV resistance;    オオムギ縞萎縮病(BaYMV)抵抗性;    Pyramiding;    集積法;   

DOI  :  10.1626/jcs.77.174

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

BaYMV resistance genes, rym5 and rym3, were pyramided using esterase isozyme genes (Est1-Est2-Est4), whose loci had been reported to be tightly linked with rym5. BaYMV reaction was also evaluated in the field contaminated with BaYMV strain I (to which cultivars with rym5 and rym3 are resistant) or III (to which cultivars with rym5 is susceptible). Malting barley cultivars having rym5 with esterase isozyme pattern Ca-null-Nz (Mokusekko-type) were crossed with the cultivars having rym3 with Pr-Fr-Su (Prior-type). Selection of resistant lines in the field contaminated with BaYMV I with the esterase isozyme genotype of Pr-Fr-Su (Prior-type) gave lines with rym3. Selection of resistant lines in the field contaminated with BaYMV III with esterase isozyme genotype of Ca-null-Nz (Mokusekko-type) gave lines pyramided with rym5 and rym3, in which the crossing-over rate was about 4.9%. During the selection by agronomic characters, the frequency of rym3 was lowered significantly.

【 授权许可】

Unknown