Organogenesis in Leaf Protoplast-Derived Calli of Mungbean

Japanese Journal of Crop Science

Iriawati¹  Takeshi TANIGUCHI¹  Hiroshi MIYAKE¹ 

关键词: callus;    カルス;    mungbean;    器官形成;    organogenesis;    根形成;    protoplast;    プロトプラスト;    root formation;    リョクトウ;   

DOI  :  10.1626/jcs.63.714

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

Protoplasts were successfully isolated from mungbean leaves (Vigna radiata L. Wilczek) using an enzyme solution containing 4% Meicelase P-1, 1% Macerozyme R-10, 1% potassium dextran sulphate and 0.5 M mannitol. Approximately 4.1×107 protoplasts per gram fresh weight were released after digestion with an enzyme for 4 hours in the dark on a shaker. The cultured protoplasts started to divide 5 days after culture followed by sustained division, and a microcolony was produced on day 15 of culture. Microcalli appeared on the medium surface a month after culture and were large enough for transfer to a callus induction medium after 1.5 month of culture. Having transferred to the regeneration media, green and compact calli were developed in MS medium supplemented with NAA and BA. Globular-like calli appeared in the MS basal medium or in the mixture of MS salts and B5 vitamin supplemented with 2, 4-D. Root formation then occurred from this calli.

【 授权许可】

Unknown