Effect of Seed Preconditioning and Culture under Different Light Intensities on Callus Induction and Regeneration in Excised Cotyledon Cultures of Mungbean (Vigna radiata (L.) Wilczek)

Japanese Journal of Crop Science

Josefina O. NARCISO¹  Yuzo FUTSUHARA¹  Kazumi HATTORI¹  Tomikichi WADA¹ 

关键词: Callus;    カルス;    Light;    再分化;    Mungbean;    前処理;    Preconditioning;    å…‰;    Regeneration;    リョクトウ;   

DOI  :  10.1626/jcs.66.67

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

Different seed preconditioning treatments and light intensities were tested for their effect on the callus induction and regeneration in cotyledon cultures of mungbean ( Vigna radiata (L.) Wilczek). There were six preconditioning treatments evaluated including imbibition in sterile distilled water, inoculation in plain agar, and inoculation in 6-benzyladenine (6-BA) supplemented media over a varing period of time. The cultures were incubated in two light intensity conditions; namely, full light (50 μmol m-2 sec-1) and minimum light (8.5 μmol m-2 sec-1). Results of the study showed that (1) plain imbibition of seeds in sterile distilled water for 16 hours was more favorable for regeneration than preconditioning with 6-BA enriched media, and (2) preconditioning of callus formation and better callus growth. Other important observation. was the differential response to plantlet regeneration under different regeneration media. Plantlet regeneration in B5 basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BA was relatively higher than the other regeneration media under full light intensity while Murashigc and Skoog (MS) basal medium and MS basal medium supplemented with 1.0 μM NAA and 9.0 μM 6-BA had highter plantlet regeneration under minimum light intensity.

【 授权许可】

Unknown