Effect of NEFA and Glucose Levels on CPT-I mRNA Expression and Translation in Cultured Bovine Hepatocytes

Journal of Veterinary Medical Science

Cheng XIA¹  Ri-he ZHANG²  Hong-you ZHANG¹  Zhe WANG²  Shi-xin FU¹  Chuang XU¹ 

[1] Department of Clinical Veterinary Medicine, Animal Science and Technology College, Heilongjiang Bayi Agricultural University;Department of Clinical Veterinary Medicine, Animal and Veterinary College, Jilin University

关键词: CPT-I;    fatty acid transport;    glucose;    hepatocytes;    NEFA;   

DOI  :  10.1292/jvms.10-0164

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(2)The objective of this study was to determine the effects of NEFA and glucose on carnitine palmitoyltransferase-I (CPT-I) mRNA expression in cultured bovine hepatocytes using real-time reverse transcription polymerase chain reaction and ELISA methods. The results indicated that CPT-I transcription increased gradually, but that CPT-I translation was not significantly changed, with glucose concentrations ranging from 0 to 3.0 mmol/L (P<0.01). Furthermore CPT-I transcription and translation were enhanced significantly when the NEFA concentrations increased from 0 to 1.2 mmol/L and decreased significantly when the NEFA concentrations increased from 1.2 to 4.8 mmol/L (P<0.01). A high concentration NEFA was found to reduce fatty acid oxidation, potentially explaining the development from NEB to ketosis in dairy cows.

【 授权许可】

Unknown