Detection and Genotype of Encephalitozoon cuniculi DNA from Urine and Feces of Pet Rabbits in Japan

Journal of Veterinary Medical Science

Madoka ICHIKAWA-SEKI¹  Masami KIMURA¹  Kenji YAGITA²  Kayoko MATSUO³  Mikiko AOKI¹  Tadashi ITAGAKI¹ 

[1] Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka 020–8550, Japan;Department of Parasitology, National Institute of Infectious Diseases, 1–23–1 Toyama, Shinjuku, Tokyo 162–8640, Japan;Pet Sun Co., Ltd., 1–5–3 Takamiya, Neyagawa, Osaka 572–0806, Japan

关键词: Encephalitozoon cuniculi;    genotype;    Japan;    nested-PCR;    pet rabbits;   

DOI  :  10.1292/jvms.12-0567

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)A newly developed nested polymerase chain reaction (PCR) assay targeting the internal transcribed spacer (ITS) region of the ribosomal DNA was applied to detect and characterize Encephalitozoon cuniculi DNA from pet rabbits in Japan. The analysis was carried out using 257 urinary samples and 314 fecal samples collected from 307 pet rabbits in the age group of 1 month to 12 years from 30 different prefectures of Japan and 107 fecal samples and 3 urinary samples collected from 1-month-old rabbits from 3 breeding facilities in Japan. We detected 840-bp amplicons in 20 urinary samples (7.78%) from the pet rabbits of the 13 prefectures and in 1 urinary (33.3%) and 6 fecal (5.6%) samples from the rabbits of the 2 breeding facilities. The sequences (803 bp) of the 27 amplicons had no variations and completely coincided with the sequence of E. cuniculi genotype I. To the best of our knowledge, this is the first report on the detection and genotype characterization of E. cuniculi DNA from pet rabbits in Japan.

【 授权许可】

Unknown