Bovine Leukemia Virus High Tax Molecular Clone Experimentally Induces Leukemia/Lymphoma in Sheep

Journal of Veterinary Medical Science

Norihiro NAKAE³  Shigeru NUMAKUNAI³  Kosuke OKADA³  Satoru KONNAI¹  Masanobu GORYO³  Misao ONUMA¹  Shan-ai YIN³  Masahiko TAKAHASHI⁴  Shin-nosuke TAKESHIMA⁴  Manabu IKEDA³  Yoko AIDA⁴  Konomi KURAMOCHI³  Shigeaki TAKAMI³  Shigeru TAJIMA⁴  Tou-ichi HIRATA² 

[1] Graduate School of Veterinary Medicine, Hokkaido University;Field Science Center, Faculty of Agriculture, Iwate University;Department of Veterinary Pathology, Faculty of Agriculture, Iwate University;Retrovirus Research Unit, RIKEN

关键词: bovine leukemia virus;    lymphoma;    molecular clone;    sheep;    tax;   

DOI  :  10.1292/jvms.67.1231

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Sheep were inoculated with high tax coded pBLV-IF (H group, Nos.1-5) of bovine leukemia virus (BLV), wild tax coded pBLV-IF (W group, Nos. 6-11), or control plasmid (C group, Nos. 12-14). During the observation period (4 to 46 months), 5 of 5 cases in H group and 3 of 6 cases (Nos. 6, 7, 9) in W group became positive for gp 51. Only 1 case in H group became leukemic, and one case each of H and W groups developed lymphoma. In No. 3, lesions were found in multiple organs including the lymph nodes, gastrointestinal tract following abomasum, and heart. In No. 6, lesions of lymphoma were found only in the jejunum and heart. Morphologically, small to middle-sized lymphocytic neoplastic (NP) cells were found in both cases, but lymphoblastic NP cells were found only in No. 3. By immunohistochemical examination, the phenotypes of NP cells were determined as CD1-, CD4-, CD5--, CD8α-, sIgM+, λ light chain+, B-B4+, MHC class II+ in both case. The results of this study indicate that inoculation of pBLV-IF can induce lymphocytic and lymphoblastic leukemia/lymphoma in sheep. Additionally, it is suggested that the expression rate of tax gene is not associated with the development of leukemia/lymphoma in sheep experimentally inoculated with pBLV-IF.

【 授权许可】

Unknown