The changes of prooxidant and antioxidant enzyme activities in bovine leukemia virus‐transformed cells Their influence on quinone cytotoxicity

FEBS Letters

Nemeikait≐, Ausra¹  C̆≐nas, Narimantas² 

[1] Institute of Immunology of Lithuanian Academy of Sciences, Mokslininky 12, 2600 Vilnius, Lithuania;Institute of Biochemistry of Lithuanian Academy of Sciences, Mokslininky 12, 2600 Vilnius, Lithuania

关键词: Bovine leukemia virus;    Cell transformation;    Quinone cytotoxicity;    BLV;    bovine leukemia virus;    FLK and Mi-18;    BLV-transformed lamb and calf embryo kidney fibroblast lines;   

DOI  :  10.1016/0014-5793(93)81762-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It was found that the activities of prooxidant enzymes (NAD(P)H oxidases and NAD(P)H:cytochrome c reductases) in bovine leukemia virus-transformed calf and lamb embryo kidney fibroblasts (lines Mi-18 and FLK) were by 1.25–18 times higher when compared to corresponding nontransformed calf cells. The activity of DT-diaphorase was also increased by about one order of magnitude in transformed cells. The activities of antioxidant enzymes were almost unchanged (superoxide dismutase), decreased by 13% or 53% (catalase) or increased by 25% or 90% (glutathione reductase) in Mi-18 or FLK cells, respectively. These changes of enzyme activity increased the toxicity of simple redox-cycling quinones (duroquinone, naphthazarin) towards transformed cells, but did not affect the toxicity of daunorubicin. The latter was most probably related to the inhibition of plasma membrane NADH dehydrogenase.

【 授权许可】

Unknown