Monoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon

Diseases of Aquatic Organisms

Paisarn Sithigorngul¹  Sombat Rukpratanporn¹  Weerawan Sithigorngul¹  Parin Chaivisuthangkura¹  Piamsak Menasveta¹  Siwaporn Longyant¹ 

关键词: Capsid;    Dot-blot;    Envelope;    Immunohistochemistry;    Matrix;    Monoclonal antibodies;    Penaeus monodon;    Viral infection;    YHV;   

DOI  :  10.3354/dao049071

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Monoclonal antibodies specific to 22, 67 and 135 kDa proteins of yellow-head virus (YHV) were produced from a mouse immunized with partially purified YHV isolated from the haemolymph of experimentally YHV-infected Penaeus monodon. Fourgroups of monoclonal antibodies were identified. One group of antibodies bound only to native protein of YHV while the others were specific to 135, 67 and 22 kDa proteins in both native and denatured forms. All antibodies could be used to detect YHVinfection by means of dot blot and immunohistochemistry. However, antibodies specific to the 22 kDa protein gave the best immunohistochemistry results in terms of intensity and sharpness of staining.

【 授权许可】

Unknown