Development of Polymerase Chain Reaction and Comparison with In situ Hybridization for the Detection of Haemophilus parasuis in Formalin-Fixed, Paraffin-Embedded Tissues

Journal of Veterinary Medical Science

Yoonchul HA²  Sung-Hoon KIM¹  Kwonil JUNG²  Chanhee CHAE² 

[1] Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University;Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University

关键词: DNA extraction;    Haemophilus parasuis;    in situ hybridization;    PCR;   

DOI  :  10.1292/jvms.66.841

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Cited-By(7)DNA extraction and nested polymerase chain reaction (PCR) were developed for the detection of Haemophilus parasuis from formalin-fixed, paraffin-embedded tissues. The results for nested PCR were compared with those determined by in situ hybridization. The optimal results obtained show that use of xylene deparaffinization, digestion with proteinase K followed by nested PCR is a reliable detection method. A distinct positive signal was detected in 20 pigs naturally infected with H. parasuis by in situ hybrdization. The rate of agreement between nested PCR and in situ hybridization for the detection of H. parasuis in formalin-fixed, paraffin-embedded tissues was 100%. The nested PCR could be applied successfully to formalin-fixed, paraffin-embedded tissues for the detection of H. parasuis with bacterial isolation.

【 授权许可】

Unknown