High-Performance Liquid Chromatographic Determination of Ustiloxin A in Forage Rice Silage

Journal of Veterinary Medical Science

Tsutomu UCHIHARA¹  Kazuhide MORIMOTO²  Shigeru MIYAZAKI¹  Yuichi MATSUMOTO¹ 

[1] Safety Research Team, National Institute of Animal Health, NARO;Livestock Technology Research Center, Hiroshima Prefectural Technology Research Institute

关键词: Forage Rice;    HPLC;    Silage;    Ustiloxin A;   

DOI  :  10.1292/jvms.71.239

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(7)Cited-By(10)We describe a high-performance liquid chromatographic method for determining ustiloxin A, a mycotoxin produced by Ustilaginoidea virens, in forage rice silage. Lyophilized silage samples were ground and extracted with water. The extracts were purified by solid-phase extraction and subjected to high-performance liquid chromatography using an octadecylsilane-bonded column. Separated ustiloxin A was detected with ultraviolet (UV) absorption at 254 nm. The limit of quantitation for ustiloxin A in silage found to be 2.5 mg/kg. The present method can be used for routine monitoring of the contamination of ustiloxin A in forage rice silage.

【 授权许可】

Unknown