A monoclonal antibody-based enzyme-linked immunosorbent assay for detection of ustiloxin A in rice false smut balls and rice samples

卷:181

Fu, Xiaoxiang ; Wang, Xiaohan ; Cui, Yongliang ; Wang, Ali ; Lai, Daowan ; Liu, Yang ; Li, Qing X. ; Wang, Baomin ; Zhou, Ligang

关键词: Ustiloxin A;    icELISA;    Mycotoxin;    Phytotoxin;    Rice false smut;    Villosiclava virens;   

DOI  :  10.1016/j.foodchem.2015.02.068

学科分类：食品科学和技术

【 摘 要 】

Ustiloxin A, a cyclopeptide mycotoxin, was isolated from the pathogenic fungus Villosiclava virens that causes rice false smut, a worldwide devastating rice disease. A monoclonal antibody (mAb) 2D3G5 was generated with ustiloxin A-bovine serum albumin conjugate. A highly sensitive and specific indirect competitive enzyme-linked immunosorbent assay (icELISA) was then developed. It possessed a median inhibition concentration (IC50) of 13.8 ng/mL and a working range of 2.8-72 ng/mL. The mAb 2D3G5 recognized ustiloxin B with the cross-reactivity as 4%. The average recoveries of ustiloxin A from rice false smut balls and peeled rice samples ranged from 92% to 117% and from 92% to 107%, respectively. Comparison of the concentrations of ustiloxin A in rice false smut balls detected by both icELISA and high performance liquid chromatography-photodiode array detection indicated that the developed icELISA was suitable for detection of ustiloxin A in rice food and feed samples. (C) 2015 Elsevier Ltd. All rights reserved.