In Vitro Inhibition of Cholera Toxin Production in Vibrio cholerae by Methanol Extract of Sweet Fennel Seeds and Its Components

Japanese Journal of Infectious Diseases

T. Ramamurthy¹  Atsushi Hinenoya³  Sharda Prasad Awasthi³  Shunji Aoki²  Emiko Iwaoka²  Masahiro Asakura³  M. Shamim Hasan Zahid³  Shinji Yamasaki³  Nityananda Chowdhury³  Shruti Chatterjee³ 

[1] National Institute of Cholera and Enteric Diseases;Faculty of Pharmacy, Hyogo University of Health Sciences;Graduate School of Life & Environmental Sciences, Osaka Prefecture University

关键词: sweet fennel seed;    cholera toxin;    trans-anethole;    4-allylanisole;    Vibrio cholerae;   

DOI  :  10.7883/yoken.JJID.2015.421

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(30)A newly emerged Vibrio cholerae O1 El Tor variant strain with multidrug resistance is considered a threat to public health. Recent strategies to suppress virulence factors production instead of bacterial growth may lead to less selective pressure for the emergence of resistant strains. The use of spices and their active constituents as the inhibitory agents against cholera toxin (CT) production in V. cholerae may be an alternative approach to treat cholera. In this study, we examined the potential of sweet fennel seed (Foeniculum vulgare Miller var. dulce) methanol extract to inhibit CT production in V. cholerae without affecting viability. The methanol extract of sweet fennel seeds significantly inhibited CT production in various V. cholerae strains, regardless of serogroup or biotype. Interestingly, trans-anethole and 4-allylanisole, essential oil components of sweet fennel seeds, also demonstrated similar effects. Here, we report that sub-bactericidal concentrations of sweet fennel seed methanol extract and its major components can drastically inhibit CT production in various V. cholerae strains.

【 授权许可】

Unknown