Excitotoxicity-Associated p53 Expression in Adult Rat Retina Is Mediated by Calpain Activity but Not by Cl− Influx

Journal of Pharmacological Sciences

Yasuhiro Hama¹  Akinori Akaike¹  Toshiaki Kume¹  Yasuhiko Izumi¹  Hiroshi Katsuki² 

[1] Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan;Department of Chemico-Pharmacological Sciences, Graduate School of Pharmaceutical Sciences, Kumamoto University, Japan

关键词: apoptosis;    calcium;    retina;   

DOI  :  10.1254/jphs.09105SC

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)We have previously demonstrated an important role of influx of Cl− rather than Ca2+ in acute excitotoxicity in adult rat retina. As p53 has been implicated in delayed apoptotic cell death, here we examined the appearance of p53 immunoreactivity in a rat eyecup preparation. Kainate induced p53 expression in a subpopulation of ganglion cells and other cells in the ganglion cell layer. Application of a calpain inhibitor, but not reduction of extracellular Cl−, markedly inhibited kainate-induced p53 expression. The results suggest that, in contrast to acute excitotoxicity, delayed excitotoxicity in retinal neurons is mediated by Ca2+-dependent processes, including calpain activation.

【 授权许可】

Unknown