Detection of Staphylococcus aureus using the REP-PCR technique to monitor goat milk quality

Revista Brasileira de Zootecnia

Villarroel, Arturo Bernardo Selaive⁴  Alves, Francisco Selmo Fernandes¹  Fernandes, Francisco Eden Paiva²  Chapaval, Lea¹  Sousa, Francisca Geovania Canafístula de³  Olivindo, Cellyneude de Souza² 

[1] Embrapa Caprinos, Sobral;Universidade Federal de Viçosa, Viçosa;Universidade Estadual Vale do Acaraú, Sobral;Universidade Federal do Ceará, Fortaleza

关键词: fingerprints;    management;    molecular epidemiology     IntroduçãoO sistema APPCC (análise de perigos e pontos críticos de controle) tem sido útil para avaliar os riscos de perigos e estabelecer mecanismos ou medidas de controle visando reduzir os riscos de contaminação física;    química ou microbiológica (Heeschen et al;   

DOI  :  10.1590/S1516-35982009000700022

学科分类：兽医学

来源: Universidade Federal de Vicosa

【 摘 要 】

The objective of the present study was to apply REP-PCR sequences in the monitoring goat milk quality, by detecting Staphylococcus aureus, in samples of from milker hands, goat teats, milk, milking machine and water, for the future establishment and implantation of the system of Hazard Analysis Critical Control Points (HACCP). Several fingerprints were verified of all the isolates collected from the different sources studied (milker hands, goat teats, milk, milking machine and water). Very similar band behaviors were observed that indicated that the isolates can be reported as epidemic clones. Milker´s hands were was characterized as a critical control point (CCP), because it stands out as an initial point of contaminationin the Staphylococcus aureus samples. The technique was shown to be efficient for the similarity analysis among individuals of the Staphylococcus aureus species, and is therefore a useful tool for investigation of management faults and consequently, in the search for more efficient control to prevent or minimize the spread of pathogenic microorganisms that cause serious illnesses in humans and animals that can be transmitted through products such as milk and its products.

【 授权许可】

CC BY-NC