FINGERPRINT ANALYSIS OF UNFRACTIONATED Piper PLANT EXTRACTS BY HPLC-UV-DAD COUPLED WITH CHEMOMETRIC METHODS

Journal of The Chilean Chemical Society

DELGADO, WILMAN A¹  CUCA, LUIS E¹  BERNAL, FREDDY A¹ 

[1] Universidad Nacional de Colombia, Bogotá, Colombia

关键词: Piper;    Piperaceae;    fingerprints;    HPLC;    chemometrics;    PCA;    pest control potential;   

DOI  :  10.4067/S0717-97072012000300013

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

23 crude ethanolic extracts of Piper plants were analyzed by combining HPLC fingerprints and chemometric methods including principal component analysis (PCA), hierarchical clustering analysis (HCA) and parallel factor analysis (PARAFAC). All statistical analysis were carried out both with and without previous preprocessing by counting with alignment of the fingerprints by correlation optimized warping (COW) and several normalization methods. Normalization processes of autoscaling, range scaling and vast scaling resulted to be the better offering the most relevant information. Both direct two-way methods (PCA and HCA) and HCA after data minimization employing a three-way method (PARAFAC) were demonstrated to be useful in the establishment of chemical composition relationships among Piper samples, and resulting in agreement with phylogenic relationships previously reported in literature to some Piper species.

【 授权许可】

Unknown