Effect of Medium Change on the Development of In Vitro Matured and Fertilized Bovine Oocytes Cultured in Medium Containing Amino Acids

Journal of Veterinary Medical Science

Seiji KATAGIRI¹  Yoshiyuki Takahashi¹  Koji IKEDA¹ 

[1] Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan

关键词: ammonium;    culture;    embryo;   

DOI  :  10.1292/jvms.62.121

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(7)Bovine in vitro matured and fertilized oocytes were cultured for 153 hr in groups of 3 or 30 in 30 μl of modified synthetic oviduct fluid medium supplemented with amino acids. The concentration of ammonium in culture medium at 153 hr of culture was significantly decreased by medium change at 72 hr of culture. However, regardless of embryo density, medium change had no beneficial or detrimental effect on the development of bovine embryos. Increase in the development to blastocysts and production of ammonium were observed when embryos were cultured in groups of 30. These results indicated that the ammonium concentration detected in this culture system has a negligible effect on the development of bovine embryos to blastocysts.

【 授权许可】

Unknown