In Vitro Study of Teratogenic Effects of Caffeine on Cultured Rat Embryos and Embryonic Cells

Journal of Veterinary Medical Science

Yasuo IKEDA¹  Naoki OHYAMA¹  Takayuki IWASE¹  Masako YAMAMOTO¹  Keiko INAZAWA¹  Mitsuyuki SHIRAI¹  Yumiko IWASE¹  Hiroaki SOMIYA¹  Kazuyoshi ARISHIMA¹  Yasunobu EGUCHI¹ 

关键词: caffeine;    rat;    whole;    embryo;    culture;   

DOI  :  10.1292/jvms.56.619

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(16)Cited-By(3)The teratogenic potential of caffeine was examined in vitro by a whole embryo culture system (WECS) and an embryonic cell culture system (mi(:romas teratogen assay: MTA) in the rat. In the WECS, hyperemia of the tail, and a reducion of the placental size was induced by caffeir]e at concentrations higher than 50 pg/ml; hypoplasia of the forelimb bud was induced at concentrations higher than lOO /Lg/ml; hematoma in the yolk sac and dysmorphogenesis of the fore- and hind-limb buds, prosencephalon and tail were induced by 200 pg/ml caffeinc:. In the MTA, even with 200 pg/ml caffeine, the toxicological parameters obtained by proliferation and differentiation assays of the midbrain and limb bud cells were almost the same as in the contro1. In conclusion, caffeine induced various morPho1o9ical anomalies, but did not affect Proliferation or differentiation of cells in these experimental systems.

【 授权许可】

Unknown