PCR Detection of Anaplasma phagocytophilum and Borrelia burgdorferi in Ixodes persulcatus Ticks in Mongolia

Japanese Journal of Infectious Diseases

Yosaburo Oikawa³  Shou Masuda⁵  Toshiyuki Masuzawa⁵  Nobuhiro Takada⁴  Takashi Fukui⁵  Fubito Ishiguro¹  Jantsandoo Bataa²  Yoshihiro Okamoto⁵ 

[1] Fukui Prefectural Institute of Public Health;Mongolian National Investigation Center for Infectious Diseases;Laboratory of Medical Zoology, Kanazawa Medical University;Senior Fellow Office, Faculty of Medical Sciences, Fukui University;Laboratory of Microbiology and Immunology, Faculty of Pharmacy, Chiba Institute of Science

关键词: Anaplasma phagocytophilum;    Borrelia burgdorferi;    Ixodes persulcatus;    Mongolia;   

DOI  :  10.7883/yoken.67.47

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(26)Cited-By(2)A molecular epidemiological survey was conducted to identify the tick-borne disease agents Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Selenge Province, Mongolia. The survey was in response to a suspected A. phagocytophilum infection in a patient. In 2012, a total of 129 questing Ixodes persulcatus adult ticks were sampled by flagging vegetation. A. phagocytophilum and Borrelia spp. were detected by PCR, targeting the 16S rDNA (rrs) and 5S–23S intergenic spacer region, respectively. Infection rates for A. phagocytophilum and B. burgdorferi sensu lato spp. were 6.2% and 55.0%, respectively. Six of the 129 ticks (4.9%) were coinfected with A. phagocytophilum and B. burgdorferi sensu lato. Among Borrelia spp., the highest prevalence rate was that for B. garinii 20047 type (26.3%), followed by B. afzelii (7.8%) and B. garinii NT29 type (7.0%). Furthermore, ticks were detected that were dually infected with B. afzelii and B. garinii 20047 type (7.8%) and B. garinii NT29 and 20047 types (6.2%).

【 授权许可】

Unknown