Artificial Control of Subtype-Specific Platelet-Derived Growth Factor–Receptor Signaling

Journal of Pharmacological Sciences

Kenzo Hirose¹  Masayuki Isa¹  Yusaku Ohta¹  Shigeyuki Namiki¹ 

[1] Department of Neurobiology, Graduate School of Medicine, The University of Tokyo, Japan

关键词: platelet-derived growth factor receptor;    chimeric receptor;   

DOI  :  10.1254/jphs.09136SC

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(13)Cited-By(2)Platelet-derived growth factor (PDGF) signaling controls various physiological functions via two receptor subtypes: PDGF receptor (PDGFR) α and PDGFRβ. Nevertheless, our understanding of their roles is limited because of a lack of pharmacological tools to discriminate subtype-specific signaling. We developed a chimeric receptor by combining ligand-binding-domain truncated PDGFRβ with anti-fluorescein single chain antibody, expecting the control of PDGFRβ-specific signaling by oligomerized fluorescein as an artificial agonist. Results show that calcium mobilization, Cdc42 activation, and cell migration were elicited specifically by the artificial ligand in cells expressing the chimeric receptor. Our method is expected to be useful to understand the subtype-specific roles of PDGFRs in various cellular functions.

【 授权许可】

Unknown