Journal of Pharmacological Sciences | |
Characterization of Forskolin-Induced Ca2+ Signals in Rat Olfactory Receptor Neurons | |
Ken-ichi Otsuguro3  Toshiyuki Saito2  Shree Hari Gautam1  Shigeo Ito3  Yoshiaki Habara1  | |
[1] Laboratory of Physiology, Graduate School of Veterinary Medicine, Hokkaido University;Animal Neurophysiology Laboratory, National Institute of Agrobiological Sciences;Laboratory of Pharmacology, Graduate School of Veterinary Medicine, Hokkaido University | |
关键词: olfactory receptor neuron; forskolin; Ca2+ signal; phosphodiesterase; Ca2+ channel; | |
DOI : 10.1254/jphs.FP0040883 | |
学科分类:药学 | |
来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society | |
【 摘 要 】
References(36)Cited-By(7)Forskolin-induced Ca2+ signals were examined in isolated rat olfactory receptor neurons (ORNs) using a Ca2+ indicator, fura-2. In the soma of the ORNs, forskolin caused an increase in the intracellular Ca2+ concentration ([Ca2+]i) that was enhanced by a phosphodiesterase (PDE) 1 inhibitor, 8-methoxymethyl-3-isobutyl-1-methyl-xanthine, but not a PDE4 inhibitor, rolipram. Forskolin-induced Ca2+ signals were abolished with the removal of extracellular Ca2+ and un-affected by treatment with thapsigargin or caffeine plus ryanodine. Niflumic acid, a Ca2+-activated Cl− channel inhibitor, or nifedipine, an L-type Ca2+ channel inhibitor, slowed the initial rate of the increase in [Ca2+]i in response to forskolin. Nifedipine did not affect the increase in [Ca2+]i that was slowed by niflumic acid. In Ca2+ measurements with a confocal microscope and a calcium indicator, Fluo-4, the onset of the response to forskolin in the knob region occurred simultaneously or earlier, but not later, than that in the soma. It is suggested that the forskolin-induced Ca2+ signals are due to Ca2+ influx, but not the release of Ca2+ from Ca2+ stores, and that the initial rapid increase in [Ca2+]i is associated with the activation of the voltage-dependent Ca2+ channels in rat ORNs.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201911300929746ZK.pdf | 286KB | download |