期刊论文详细信息
Journal of Veterinary Medical Science
First Detection of Cryptosporidium baileyi in Ruddy Shelduck (Tadorna ferruginea) in China
Hongxuan HE1  Said AMER1  Chengmin WANG1 
[1] National Research Center for Wildlife Born Diseases, Institute of Zoology, Chinese Academy of Sciences
关键词: China;    Cryptosporidium baileyi;    Ruddy Shelduck;    Tadorna ferruginea;   
DOI  :  10.1292/jvms.09-0515
学科分类:兽医学
来源: Japanese Society of Veterinary Science
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【 摘 要 】

References(32)Cited-By(6)Fecal samples from Ruddy Shelduck, Tadorna ferruginea, were screened microscopically for Cryptosporidium oocysts. Five samples out of 148 ones (3.38%) were confirmed to be positive. DNA was extracted individually from positive samples and used for PCR amplification of SSU rDNA and HSP70 gene loci. The obtained PCR products were cloned in E. coli (TG1 strain) using pMD18-T vector and sequenced using standard methods. Microscopical and molecular analyses identified the obtained isolates as Cryptosporidium baileyi. To our knowledge, this is the first report of detection of C. baileyi from Ruddy Shelduck, Tadorna ferruginea, in China.

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