タンパク質立体構造情報を利用した機能予測法FCANAL：酵素・結合タンパク質への応用

Chem-Bio Informatics Journal

宮崎 智²  山登 �?郎¹  鈴木 歩¹  安藤 格士¹ 

[1] 東京理科大学�?基礎工学研究科�??生物工学専攻;東京理科大学�?薬学部�??薬学科

关键词: protein function prediction;    タンパク質機能予測;    enzyme;    酵素タンパク質;    binding protein;    結合タンパク質;    protein local structures;    タンパク質局�?立体構�?�;    amino acid propensity;    アミノ酸使用傾向;    distance distribution;    距離分布;    bioinformatics;    バイオインフォマティクス;   

DOI  :  10.1273/cbij.5.39

学科分类：生物化学/生物物理

来源: Chem-Bio Informatics Society

【 摘 要 】

References(31)Structural genomics projects are beginning to produce protein structures with unknown functions, thereby creating a need for high-throughput methods to predict functions. Although sequence-based function prediction methods have been used extensively, structure-based prediction is believed to provide higher specificity and sensitivity because functions are closely related to the three-dimensional structures of functional sites, which are more strongly conserved during evolution than sequence. We have developed FCANAL, a method to predict functions using a score matrix obtained from the distances between Cα atoms and frequencies of appearance [1]. The previous report used key residues predicted from sequence comparisons (motifs). In this report, we have expanded the method to include enzymes and binding proteins with key residues predicted on the basis of three-dimensional structures. Using FCANAL, we constructed score matrices for 31 enzymes. When we applied them to all of the structure entries deposited in the Protein Data Bank, FCANAL could detect functional sites with high accuracy. This suggests that FCANAL will help identify the functions of newly determined structures and pinpoint their functionally important regions.

【 授权许可】

Unknown