Identity of Naegleria strains isolated from organs of freshwater fishes

Diseases of Aquatic Organisms

H. Pecková¹  M. Oborník¹  I. Dyková¹  J. Lukes¹  I. Kyselová¹ 

关键词: Naegleria spp.;    Fish strains;    Molecular taxonomy;    SSU rRNA;   

DOI  :  10.3354/dao046115

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Eighteen Naegleria strains were isolated from organs of freshwater fishes belonging to 5 species. Morphometric study allowed the separation of the Naegleria strains from the non-vahlkampfiid amoeboflagellates, but was inadequatefor species determination. Six strains, representatives of groups that had a slightly different cyst size, were selected and corresponding derived clones were subjected to sequence analysis and riboprinting restriction fragment length polymorphism(RFLP)-PCR analysis of the small subunit (SSU) rRNA genes. One strain isolated from the brain of a fish with systemic infection was characterised by an intronless 2 kb long SSU rRNA gene and was identified as N. australiensis. Another 5 strains hada 1.3 kb long group I intron in their SSU rRNA gene and, based on the SSU rRNA sequences and riboprints, RFLP-PCR patterns appeared in phylogenetic trees to be closely related to Naegleria clarki.

【 授权许可】

Unknown