Persorption of Luminal Antigenic Molecule and Its Specific Antibody via Apoptotic Epithelial Cells of Intestinal Villi and Peyer's Patches into Peripheral Blood in Rats

Journal of Veterinary Medical Science

Tetsurou INAMOTO¹  Masahito TSUBATA²  Keigi CHIN¹  Sachiko ONISHI¹  Midori YUJI¹  Wang-Mei QI¹  Nobuhiko HOSHI¹  Hiroshi KITAGAWA¹  Katsuhiko WARITA¹  Toshifumi YOKOYAMA³ 

[1] Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University;Department of Animal Science, Graduate School of Science and Technology, Kobe University;Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

关键词: antigen;    apoptosis;    epithelial cell;    persorption;    specific antibody;   

DOI  :  10.1292/jvms.68.1297

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(48)Cited-By(10)The possibility of persorption of bovine serum albumin (BSA) molecules from mucous epithelial cells and its mechanism were investigated in rats orally pre-immunized by BSA for 14 consecutive days. In the small and large intestines, both the BSA antigen (BSA-Ag) and its specific antibody (SpAb) were absorbed by the epithelial cells at the late apoptotic stage (ApoEp), and were subsequently transcytosed by membranes of the small vesicles. The basal cytoplasms containing highly-concentrated BSA-Ag and SpAb were occasionally fragmented into small cytoplasmic droplets that were secreted into the lamina propria. In Peyer's patches, both BSA-Ag and SpAb were more actively absorbed and transcytosed toward the dome area by the ApoEp of the dome apex than by the M cells. BSA-Ag and SpAb were finally persorbed into the portal blood and lymph, but were never secreted into the bile. They were also engulfed by macrophage-like cells in the villous lamina propria, mesenteric lymph node and spleen, and by hepatocytes in the liver. These findings suggest that sensitized soluble luminal antigens are taken up by ApoEp in the small intestine and are finally persorbed into the peripheral blood. The uptake of luminal antigen might be mediated by its luminal SpAb.

【 授权许可】

Unknown