Reactivities of Feline Calicivirus Field Isolates with Monoclonal Antibodies Detected by Enzyme-Linked Immunosorbent Assay

Journal of Veterinary Medical Science

Tomoko TAJIMA²  Yukinobu TOHYA¹  Yuka TAKEDA²  Shunji SUGII² 

[1] Department of Veterinary Microbiology, Faculty of Agriculture, Kagoshima University;Department of Veterinary Microbiology, College of Agriculture, Osaka Prefecture University

关键词: ELISA;    feline calicivirus;    monoclonal antibody;   

DOI  :  10.1292/jvms.60.753

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Cited-By(3)Reactivities of feline calicivirus (FCV) field isolates with monoclonal antibodies (MAbs) were examined by enzyme-linked immunosorbent assay (ELISA). The reactivities of the viruses in ELISA were different from our previous results using the neutralization tests (NT). Many isolates were positive in ELISA with MAbs which recognized neutralizing epitope 3B and/or 4. However, most were negative in NT in our previous study. After absorption of two FCV strains with host cells, the non-infectious virus fluid still reacted with MAb, which recognized epitope 3B and/or 4 in ELISA. These results indicated the possibility that neutralizing epitopes are expressed on non-infectious virus particles or exist as proteinaceous molecules in virus fluid.

【 授权许可】

Unknown