Differentiation of Hamster Liver Oval Cell Following Clonorchis sinensis Infection

Journal of Veterinary Medical Science

Su-Youn JUNG⁴  Byung-Il YOON²  Kwon HUR⁴  Dae-Yong KIM⁴  Jae-Hyun LEE¹  Kyung-Hwan JOO³  Yong-Soon LEE⁵ 

[1] Experimental Pathology Laboratory, Korea Cancer Center Hospital;Division of Cellular and Molecular Toxicology, National Institute of Health Sciences;Department of Parasitology, College of Medicine, Korea University;Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University;Department of Public Health, College of Veterinary Medicine, Seoul National University

关键词: 2-acetylaminofluorene;    diethylnitrosamine;    hamster;    oval cell;    Clonorchis sinensis;   

DOI  :  10.1292/jvms.62.1303

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(6)Oval cells which appear in the liver after hepatic injuries are suspected to be progenitor cells for both hepatocytes and bile duct cells. Oval cell isolated from the livers of the hamsters treated with diethylnitrosamine and 2-acetylaminofluorene and infected with Clonorchis sinensis (CS), cultured for 2 weeks and evaluated for differentiation and plasticity by electron microscopy and immunohistochemistry. In the CS-uninfected group, glycogen granules and peroxisomes were noted in the cells that were cultured for 2 weeks. Starting at 1 week postculture, immunoreactivity of the cells to cytokeratin 19 markedly decreased but that to albumin and alpha-fetoprotein gradually increased. This means that oval cells isolated from hamsters that were not infected with CS differentiated toward hepatocyte lineage. However, in the CS-infected group, cultured cells contained numerous rough endoplasmic reticulum and showed immunoreactivity that was generally in reverse to that of CS-uninfected group, meaning that cells isolated following CS infection were primed by CS and differentiated toward bile duct cell lineage. The results of this study suggested that oval cells are indeed bipolar progenitor cells for hepatocytes and bile duct cells and can differentiate toward either lineage depending upon the priming factor.

【 授权许可】

Unknown