Isolation of Liver Oval Cells from Hamsters Treated with Diethylnitrosamine and 2-Acetyl Aminofluorene

Journal of Veterinary Medical Science

Byung-Il YOON⁴  Dae-Yong KIM⁴  Kyung-Whan JOO³  Jae-Hyun LEE²  Yong-Soon LEE¹ 

[1] Public Health, College of Veterinary Medicine, Seoul National University, Suwon 441-744, Korea;Experimental Pathology Laboratory, Korea Cancer Hospital, Seoul, Korea;Department of Parasitology, College of Medicine, Korea University, Seoul, Korea;Departments of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Suwon 441-744, Korea

关键词: centrifugal elutriation;    hamster;    isolation;    oval cell;   

DOI  :  10.1292/jvms.62.255

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(5)Recently, cholangiocellular carcinoma (CCC) was successfully induced in the hamster by infecting with Clonorchis sinensis following hepatocarcinogen treatment and has been proposed as a suitable model to study the pathogenesis of human CCC. In this hamster model, oval cells are suggested to be cells of origin of CCC. More direct analysis of histogenesis of CCC would become possible if large numbers of highly purified oval cells of hamster origin are obtained. In this study, we describe successful isolation of highly purified oval cells from hamsters. Oval cells were induced by diethylnitrosamine and 2-acetylaminofluorene treatment under choline deficient diet and isolated by centrifugal elutriation method. This isolated cells were highly homogenous in size (10.9 +/- 1.1 μm in diameter) and had a high nuclear to cytoplasmic ratio, an oval-shaped nucleus and a few cytoplasmic organelles. Immunocytochemically, 85.4 +/- 1.6%, 75.1 +/- 2.0%, 62 +/- 1.5% and 25.6 +/- 2.7% of the isolated cells were positive for cytokeratin 19, OV-6, albumin and alpha-fetoprotein, respectively, indicating that these cells had phenotypic characteristics of both hepatocytes and bile duct epithelium. The isolated cells were therefore considered to be hamster oval cells.

【 授权许可】

Unknown